Nakajima 3)reported the isolation of a tetradecapeptide from the skin of Rana nigroma- culata and the determination of the amino acid sequence as bradykinyl-Val-Ala-Pro-Ala- Ser-OH(I).We carried out the synthesis of I in order to confirm the proposed structure
نویسندگان
چکیده
Synthesis is described of bradykinyl-Val-Ala-Pro-Ala-Ser-OH.All the fragment condensations were carried out by Rudinger's azide method in order to minimize the racemization during the coupling steps involving peptide fragments.The ensuing tetradecapeptide with a high degree of homogeneity was found to be identical with bradykinyl-ValAla-Pro-Ala-Ser-OH of natural origin with respect to chemical and biological properties.
منابع مشابه
Amino acid Substitution Mutations Analysis of gyrA and parC Genes in Clonal Lineage of Klebsiella pneumoniae conferring High-Level Quinolone Resistance
Background: Emergence Klebsiella pneumoniae resistant to quinolone antibiotics due to mutations in gyrA and parC genes created problem for treatment of patients in different hospitals in Iran. The objective of this study was to determine the amino acid substitutions of GyrA and ParC proteins in certain clonal lineages of the K. pneumoniae conferring high level quinolone resistance. Methods: One...
متن کاملMolecular characterization of apolipoprotein A-I from the skin mucosa of Cyprinus carpio
Apolipoprotein A-I is the most abundant protein in Cyprinus carpio plasma that plays an important role in lipid transport and protection of the skin by means of its antimicrobial activity. A 527 bp cDNA fragment encoding C terminus part of apoA-I from the skin mucosa of common carp was isolated using RT-PCR. After GenBank database searching, a partial sequence containing a coding sequence (CDS)...
متن کاملMolecular characterization of apolipoprotein A-I from the skin mucosa of Cyprinus carpio
Apolipoprotein A-I is the most abundant protein in Cyprinus carpio plasma that plays an important role in lipid transport and protection of the skin by means of its antimicrobial activity. A 527 bp cDNA fragment encoding C terminus part of apoA-I from the skin mucosa of common carp was isolated using RT-PCR. After GenBank database searching, a partial sequence containing a coding sequence (CDS)...
متن کاملPartial amino acid sequence of human plasma retinol-binding protein. Isolation and alignment of the five cyanogen bromide fragments and the amino acid sequences of four of the fragments.
Studies are reported on the primary structure of human retinol-binding protein (RBP), the specific plasma transport protein for vitamin A. The protein consists of a single polypeptide chain of 186-187 amino acids. RBP was cleaved by cyanogen bromide into five fragments, CB-I (27 residues), CB-11 (25 residues), CB-III (20 residues), CB-IV (15 residues), and CB-V (99-100 residues). The cyanogen b...
متن کاملThe Effect of Energy Sources and Levels on Performance and Breast Amino Acids Profile in Cobb 500 Broiler Chicks
The present study conducted to investigate the effect of energy sources and levels on performance and breast meat amino acids profile in Cobb 500 broiler chicks. A total of 600 1-day-old Cobb 500 broiler chicks with an average weight of 39 ± 0.50 g were randomly divided into five treatments. Each treatment was further divided into four replicates. Chicks were fed a basal diet based on corn and ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2010